FIG 2.

Maximum likelihood (ML) tree of Clostridium 16S rRNA gene sequences. The best ML tree computed with PhyML under the general time reversible (GTR) model of nucleotide substitution (four gamma categories) with 100 bootstraps. Bootstrap values greater than 60 are shown at their respective nodes, while nodes present in all bootstraps are indicated with an asterisk. Species/strain names are indicated before the @ symbol, and accession numbers are indicated after the @ symbol. The Clostridium sporogenes strains from this study are colored light orange. The previous Clostridium sporogenes, Clostridium botulinum, Clostridium perfringens, Clostridium sardiniense, Clostridium butyricum, Clostridium cellulovorans, and Clostridium tetani species are colored dark orange, navy, green, pink, gold, cyan, and purple, respectively. OTUs, operational taxonomic units.