FIG 6.

Effect of Mn²⁺ on extracellular ROS generation and cell survival. (A) Time-dependent monitoring of ROS in the supernatant of phage-treated (host plus phage) or untreated cells (host) using NBT. In the case of the phage-treated cells, the experiment also was performed in the presence of Mn²⁺ (host plus phage plus Mn²⁺), which was added at the 2-h time point (arrow). (B) Survival of host cells following phage treatment performed either in the absence (−) or presence (+) of Mn²⁺ at the indicated time points. Each data point represents the mean ± standard deviation derived from three biological replicate experiments.