FIG 4.

Detection of hsp18 asRNA and hsp18 mRNA in the O. oeni recombinant strains. Dot blot hybridization with oligonucleotide probe DIG-mhsp18 complementary to mRNA (A) or oligonucleotide probe DIG-ashsp18 complementary to antisense RNA (B) was performed. Recombinant strains carrying the native plasmid (Oosyn) or carrying the plasmid expressing hsp18 asRNA (OoAS18) were cultivated at 30°C in FT80m medium supplemented with appropriate antibiotics until the end of the exponential growth phase. Total RNA was isolated, and 5 μg of RNA from each strain and each condition was loaded on a nitrocellulose membrane.