TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or characteristicsa | Source or reference |
|---|---|---|
| Escherichia coli strains | ||
| EC101 | JM101[supE thi (lac proAB) F′ traD36 proAB lacIq ZΔM15] with repA from pWV01 integrated in chromosome | Promega (Lyon, France) |
| EcAS18 | EC101 harboring pSIPSYNhsp18-ORF | This study |
| EcAS18-UTR | EC101 harboring pSIPSYNhsp18-5′ UTR | This study |
| Ecsyn | EC101 harboring pSIPSYN | This study |
| Oenococcus oeni strains | ||
| ATCC BAA-1163 | Wild-type strain, Vanr | Laboratory stock |
| OoS18 | ATCC BAA-1163 harboring pSIPSYNAShsp18-ORF | This study |
| OoAS18-UTR | ATCC BAA-1163 harboring pSIPSYNAShsp18-RBS | This study |
| Oosyn | ATCC BAA-1163 harboring pSIPSYN | This study |
| Plasmids | ||
| pGID052 | Eryr Lincor; 3.38-kb HindIII DNA fragment of pLC22R, oriR of pLC22R | 36 |
| pSYNS3 | Eryr Lincor; derived from pGID052, PSYN promoter cloned between SwaI and PvuII sites | This study |
| pSIP409 | Eryr Ori+ Rep256 sppK sppR gusA, under control of PorfX promoter from pSIP401 | 41 |
| pSIPSYN | Eryr Lincor; derived from pSIP409, deletion of sppK, sppR, and gusA, under control of PSYN promoter | This study |
| pSIPSYNAS18 | Eryr Lincor; derived from pSIPSYN, ORFhsp18 in antisense orientation under control of PSYN promoter | This study |
| pSIPSYNAS18-UTR | Eryr Lincor; derived from pSIPSYN, 5′ UTR hsp18 in antisense orientation under control of PSYN promoter | This study |
a
Vanr, vancomycin resistance; Eryr, erythromycin resistance; Lincor, lincomycin resistance.