FIG 4.

Gene silencing of ROR2 in SGHPL-4 trophoblasts. Human trophoblasts were transfected with nonsilencing RNA duplexes or siRNA duplexes that specifically target ROR2. At 24 h posttransfection, trophoblasts were infected with CMV at MOI of 1 PFU/cell or mock infected and analyzed at 3 days p.i. (A) mRNA expression was analyzed using real-time PCR analyses. Data are displayed relative to NS-treated cells (means + SEMs; ***, P < 0.001) B) Protein expression was analyzed using Western blotting. All data are representative of two independent experiments.