FIG 5.

Gene silencing ROR2 partially restores CMV-reduced migration of SGHPL-4 trophoblasts. Human trophoblasts were transfected with nonsilencing RNA duplexes or siRNA duplexes that specifically target ROR2. At 24 h posttransfection, trophoblasts were infected with CMV (■) at an MOI of 1 PFU/cell or mock infected (□). (A) Two days p.i., uninfected or infected cells were seeded in Transwells, stimulated with Wnt5a (200 ng/ml) or PBS control for 16 h, and analyzed 3 days p.i. Images are representative of three independent experiments. (B) Average migration was determined by capturing five images (15 fields per treatment) from each well and measuring the intensity using ImageJ software (means + SEMs; **, P < 0.01). (C) Proliferation was analyzed using MTT assays. Data are presented as means + SEMs and are representative of three independent experiments.