FIG 1.

Anti-H10 and anti-N8 antibodies display heterologous cross-reactivity. The minimal binding concentrations of anti-H10 (green) and anti-N8 (black) MAbs and stalk-reactive MAb 9H10 (red) to either purified whole virus (coated at 5 μg/ml) or recombinant, baculovirus-expressed proteins (coated at 2 μg/ml) as measured by ELISA are shown. All 18 MAbs showed high reactivity to purified JD13 (H10N8) virus (A), whereas only the HA-directed MAbs displayed reactivity to an H10N7 virus (A/mallard/Interior Alaska/10BM01929/10) (B) and only the NA-directed MAbs displayed reactivity to an H3N8 virus (A/Northern shoveler/Alaska/7MP1708/07) (C). These findings were confirmed by quantitative ELISAs to purified, recombinant H10 and N8 proteins from either the matched JD13 (H10N8) virus or the divergent strains (D to I) from both the Eurasian and North American lineages.