FIG 2.

GLA cross-linking of BG505 trimers. The trimers were cross-linked with 7.5 mM GLA, and their antigenicity was tested by capture ELISA. (A) Representative titration curves from a capture ELISA are shown for selected antibodies. Error bars indicate the SD of results from replicate wells. Data are representative of two to six independent experiments. (B and C) Binding indices were calculated as AUC (cross-linked)/AUC (unmodified). Values of <1 indicate that there was a reduction in antibody binding; values of 1 (dotted line) represent no change in binding. (B) Mean binding indices from at least two independent experiments are shown for all antibodies tested. (C) The antibodies are grouped into neutralizing and nonneutralizing categories. ***, P < 0.001 (Mann-Whitney U test).