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. 2015 Dec 30;90(2):813–828. doi: 10.1128/JVI.01942-15

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Copyright © 2015 Schiffner et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — EDC/NHS cross-linking of BG505 trimers. (A) Chemical structure of EDC cross-linker. (B) Reducing SDS-PAGE gel of unmodified (UM) and EDC/NHS cross-linked (EDC) BG505. (C) EDC/NHS cross-linked material was analyzed by DSC. Modeled peaks from a non-two-state fit and their sum are indicated. (D) Negative-stain EM analysis of EDC/NHS cross-linked BG505: 2D class averages are shown. (E) EDC/NHS cross-linked BG505 was analyzed by capture ELISA. Binding indices were calculated as AUC (cross-linked)/AUC (unmodified). Values of <1 indicate there was a reduction in antibody binding; values of 1 (dotted line) represent no change in binding. Mean binding indices from at least two independent experiments are shown for all MAbs tested. (F) The MAbs are grouped into neutralizing (bNAbs) and nonneutralizing (non-NAbs) categories. ***, P < 0.001 (Mann-Whitney U test).