FIG 5.

Positive and negative selection of GLA or EDC/NHS cross-linked BG505 trimers. (A and B) bNAb and non-NAb binding to positively (A, Pos) and negatively (B, Neg) selected cross-linked trimers was compared to that of unmodified (UM) and cross-linked unselected (X-link) trimers by capture ELISA. (C) Summary of changes in bNAb and non-NAb binding. Data for antibodies against the CD4bs (red), V2 loop (brown), V3 (blue), and gp41 (purple) are represented in color as indicated. Black symbols indicate other non-NAb epitopes. Values of 1 (dotted line) represent no change in binding, and values of <1 indicate reduced binding and values of >1 indicate increased binding compared to unmodified protein. (D) Model of a membrane-bound trimer (EMD-5019 and EMD-5022) with the epitopes indicated in color as described for panel C.