FIG 2.

Interaction of c-MYC with E1A. (A to C) Interaction of exogenously expressed c-MYC and MYCBP. HeLa cells were transfected with the expression vectors for Flag-MYC, EGFP, EGFP-MYC, and Flag-MYCBP. Twenty-four hours after transfection, the cells were infected with hAd5 dl312 (E1A null), 12S wt, or dl1102 (100 PFU/cell). The whole-cell lysates (WCL) were immunoprecipitated (IP) with the Flag Ab (A and B) or the E1A Ab (M73) (C). The blots were probed with the E1A Ab (M73) (A to C) or the EGFP Ab (C). (D and E) Interaction of endogenous c-MYC and MAX. (D) HeLa cells were infected with hAd5 12S wt or mutant dl1102, and the WCLs were immunoprecipitated with the E1A Ab (M73). The Western blots were probed with the Abs for pRb (IF8), c-MYC, MAX, p400, and TRRAP. (E) Interaction of c-MYC with E1A in transformed cells. The WCLs of HeLa or 293 cells were immunoprecipitated with the E1A Ab (M73), and the Western blots were probed for pRb (C15), c-MYC, and MAX.