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Dimerization of BGLF2. 293T cells were cotransfected with FLAG-tagged BGLF2 (BGLF2-Flag) and V5-tagged BGLF2, BGLF2LL-AA, or BZLF1 or with pcDNA3.1 empty vector. Twenty-four hours after transfection, cell lysates were prepared in RIPA buffer and immunoprecipitated (IP) with anti-FLAG agarose beads (A) or anti-V5 agarose beads (B). Immune complexes were Western blotted (WB) with an antibody to the V5 or FLAG epitope.
