FIG 1.

Chitin-binding assays for WSSV particles, envelope and nucleocapsid. WSSV particles (10⁹) (A), nucleocapsids (from 10⁹ virions) (B), and envelopes (from 10⁹ virions) (C) were incubated separately with 10 μl of chitin beads. The chitin-bound fractions were analyzed by Western blotting (WB) with an anti-VP28 antibody (1:10,000) and anti-VP51 antibody (1:3,000). For the oligochitosan blocking assay (D), WSSV particles (10⁹) was preincubated with 0, 1.5, 2.25, and 3 mg of oligochitosan in 400 μl of TMN buffer for 3 h. Then 10 μl of chitin beads was added to the mixture. After further incubation for 1 h, the beads were washed and the chitin-bound complexes were analyzed by Western blotting with an anti-VP28 antibody.