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The P-VP24186–200 peptide inhibits the WSSV-chitin interaction. Ten microliters of chitin beads were preincubated with 0, 0.6, 0.9, and 1.2 mg of P-VP24186–200 or P-VP24148–162 (control peptide) in 300 μl of TMN buffer for 1 h, and then WSSV (109 virions in 100 μl TMN buffer) was added to the mixture and incubated for 1 h with gentle rotation. The beads were washed five times, and the chitin-bound fraction was analyzed by Western blotting (WB) with an anti-VP28 antibody.
