FIG 1.

BZLF1 reduces TNF-α promoter activity, transcription, and expression. (A) pEGFP-BZLF1 or vector was cotransfected into 293T cells with TNF-α-luc and pRL-TK. After 24 h, 100 ng/ml TPA or HSV-1 (MOI = 10) was added, and the dual-luciferase assay was performed after 8 h of treatment. Ctr, control. (B and C) CNE-1 cells were transfected with pEGFP-BZLF1 or vector control. At 24 h after transfection, cells were left untreated or treated with HSV-1 (MOI = 10) for 8 h. The supernatants were used for the TNF-α ELISA (B), and RNA was extracted, reverse transcribed, and quantified by the inflammatory factor real-time PCR array (C). Values are shown as the means ± standard deviations of the results of three independent experiments. Statistical analysis was performed using one-way ANOVA followed by comparisons performed using the Bonferroni method. *, P < 0.05; **, P < 0.01. The arrows indicate down- or upregulation.