FIG 6.

BZLF1Δ207-210 reduces the inhibition of TNF-α and IFN-γ expression during the IE and early EBV lytic replication. (A) The control vector, BZLF1, or BZLF1Δ207-210 was cotransfected into 293T cells with LMP1-expressing plasmids and the TNF-α promoter-luciferase reporter, and the luciferase activities were determined at 24 h after transfection. (B to F) BZLF1-KO EBV BAC-harboring BJAB cells were transduced with the control, BZLF1, or BZLF1Δ207-210 using the lentiviral system (MOI = 10), the expression of viral genes was detected by Western blotting (B), TNF-α (C) and IFN-γ (E) secretions were detected by ELISA, and the relative TNF-α (D) and IFN-γ (F) mRNA levels at different time points were determined by real-time PCR. *, P < 0.05.