TABLE 1.
Primer pairs used in this study
| Target | Primer or probe sequencea |
|---|---|
| Real-time PCR primer pairs | |
| TNF-α mRNA | F: AGCCTGTAGCCCATGTTGTAG |
| R: CCGCTCGAGTCACAGGGCAATGAT | |
| IFN-γ mRNA | F: TCGGTAACTGACTTGAATGTCCA |
| R: TCGCTTCCCTGTTTTAGCTGC | |
| GAPDH mRNA | F: ACATCATCCCTGCCTCTAC |
| R: TCAAAGGTGGAGGAGTGG | |
| GAPDH genomic DNA | F: CATCATCCCTGCCTCTACTG |
| R: GCCTGCTTCACCACCTTC | |
| EBNA1 | F: CATTGAGTCGTCTCCCCTTTGGAAT |
| R: TCATAACAAGGTCCTTAATCGCATC | |
| LMP1 | F: CCACTTGGAGCCCTTTGTMTACTC |
| R: TGCCTGTCCGTGCAAATTC | |
| BALF5 | F: AACCTTTGACTCGACCATCG |
| R: ACCTGCTCTTCGATGCACTT | |
| BcLF1 | F: CATCCATGTTCATTGGGACC |
| R: CATTAGTCATACCTGCCAGG | |
| BLLF1 | F: GTCAGTACACCATCCAGAGCC |
| R: TTGGTAGACAGCCTTCGTATG | |
| BMRF1 | F: GCCGTTGAGGCCCACGTTGT |
| R: TGGGAATGGCAGGCGAGGGT | |
| EMSA probe | |
| BMLF1 | Cys5.5-CCATCACGGTCACCTTCATGAGTCAGTGCTTCGCCGGTCGCTGTGGGGCCAATCAACCGA |
| ChIP primer pairs | |
| TNF-α-ChIP | F: CCACTACCGCTTCCTCCAGATG |
| R: GGTGTGCCAACAACTGCCTTTA | |
| BMLF1-ChIP | F: CCAAACTTAGTTCAGGTGTGCCA |
| R: TAGAGTACCAGAAACACCCTCACAT | |
| EBNA1-ChIP | F: TAACGAGCAGAGGGAATGAAAG |
| R: AGGTTTCCTCAGGCTTGGCTA | |
| BSRF1-ChIP | F: GTGAGGTCAGCCGCTTCTTGG |
| R: CGTTCTGTTTAGCCCGCCGTT | |
| GAPDH-ChIP | F: CGAGGAGAAGTTCCCCAACTT |
| R: GACCCTTACACGCTTGGATGA |
a
The boldface, italic, underlined sequence indicates the ZRE site in the BMLF1 probe.