FIG 5.

IFN-γ response by M1_58–66- and NP_383–391-specific CD8⁺ T cells after stimulation with target cells transfected with various M1-NP-eGFP-encoding plasmids. Numbers of IFN-γ positive spots/10⁴ M1_58–66-specific CD8⁺ T cells (A) and NP_383–391-specific CD8⁺ T cells (B) after stimulation by target cells transfected with M1-NP-eGFP plasmids that encode the M1 protein of the avian A/H5N1 virus (WT avian) or the M1 protein of the human A/H3N2 virus (WT human) or eGFP only (mock) are shown. Data points represent means and error bars indicate SD of quadruplicates (n = 4). **, the difference between groups was statistically significant after correction for multiple hypothesis testing using an FDR of 0.01.