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(A) HIV proviruses integrate in intronic regions of transcriptionally active host genes, in the same (upper panel; sense) or opposite (lower panel; antisense) orientation with regard to local host gene transcription. (B) A closeup of the 5′ HIV region, with a schematic representation of real-time PCR assays for detection of readthrough and gag RNA. ORF, open reading frame; Ψ, HIV packaging signal (Psi).
