Skip to main content
. 2015 Dec 30;54(1):106–113. doi: 10.1128/JCM.01696-15

TABLE 3.

Results of gene amplification encoding for various virulence factorsa

Virulence gene High stringency PCR
Low stringency PCR
Biofilm producer phenotype
atlC lip sdrZ icaA icaB icaC icaD icaR fbe cna
Human isolates
    Positive strains (%) 71 93 79 79 86 86 100 100 0 0
    1 + + + + + + + +/−
    2 + + + + + + + + ++
    4 + + + + + + + + +/−
    5 +b + + + + + + + +/−
    7 + + + +/−
    8 + + + + + + +/−
    10 +b + + + + + + + +/−
    12 + + + + + + + + ++
    13 + + + + + + + + +/−
    14 + + + + + + + ++
    15 + + + + + + + + ++
    18 + + + + + + + + ++
    19 + + +/−
    138 + + + + + + + + +/−
Goat isolates
    Positive strains (%) 100 100 93 93 93 93 100 93 0 0
    23 + + + + + + + + +/−
    25 + + + + + + + + +/−
    27 + + + + + ++
    29 + + + + + + + +/−
    35 + + + + + + + + +/−
    38 + + + + + + + + +/−
    39 + + + + + + + + +/−
    42 + + + + + + + + ++
    43 + + + + + + + + ++
    44 + + + + + + + +/−
    45 + + + + + + + + +++
    46 + + + + + + + + +/−
    47 + + + + + + + + ++
    CCM + + + + + + + + ++
Total positive strains (%) 86 96 86 86 89 89 100 96 0 0
a

+, Positive; −, negative.

b

Positive for atlC gene, strain no. 5 and 10 presented an amplicon smaller than the other strains.