TABLE 3.
Results of gene amplification encoding for various virulence factorsa
Virulence gene | High stringency PCR |
Low stringency PCR |
Biofilm producer phenotype | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
atlC | lip | sdrZ | icaA | icaB | icaC | icaD | icaR | fbe | cna | ||
Human isolates | |||||||||||
Positive strains (%) | 71 | 93 | 79 | 79 | 86 | 86 | 100 | 100 | 0 | 0 | |
1 | + | + | − | + | + | + | + | + | − | − | +/− |
2 | + | + | + | + | + | + | + | + | − | − | ++ |
4 | + | + | + | + | + | + | + | + | − | − | +/− |
5 | +b | + | + | + | + | + | + | + | − | − | +/− |
7 | − | + | − | − | − | − | + | + | − | − | +/− |
8 | − | + | + | − | + | + | + | + | − | − | +/− |
10 | +b | + | + | + | + | + | + | + | − | − | +/− |
12 | + | + | + | + | + | + | + | + | − | − | ++ |
13 | + | + | + | + | + | + | + | + | − | − | +/− |
14 | − | + | + | + | + | + | + | + | − | − | ++ |
15 | + | + | + | + | + | + | + | + | − | − | ++ |
18 | + | + | + | + | + | + | + | + | − | − | ++ |
19 | − | − | − | − | − | − | + | + | − | − | +/− |
138 | + | + | + | + | + | + | + | + | − | − | +/− |
Goat isolates | |||||||||||
Positive strains (%) | 100 | 100 | 93 | 93 | 93 | 93 | 100 | 93 | 0 | 0 | |
23 | + | + | + | + | + | + | + | + | − | − | +/− |
25 | + | + | + | + | + | + | + | + | − | − | +/− |
27 | + | + | + | − | + | − | + | − | − | − | ++ |
29 | + | + | − | + | + | + | + | + | − | − | +/− |
35 | + | + | + | + | + | + | + | + | − | − | +/− |
38 | + | + | + | + | + | + | + | + | − | − | +/− |
39 | + | + | + | + | + | + | + | + | − | − | +/− |
42 | + | + | + | + | + | + | + | + | − | − | ++ |
43 | + | + | + | + | + | + | + | + | − | − | ++ |
44 | + | + | + | + | − | + | + | + | − | − | +/− |
45 | + | + | + | + | + | + | + | + | − | − | +++ |
46 | + | + | + | + | + | + | + | + | − | − | +/− |
47 | + | + | + | + | + | + | + | + | − | − | ++ |
CCM | + | + | + | + | + | + | + | + | − | − | ++ |
Total positive strains (%) | 86 | 96 | 86 | 86 | 89 | 89 | 100 | 96 | 0 | 0 |
+, Positive; −, negative.
Positive for atlC gene, strain no. 5 and 10 presented an amplicon smaller than the other strains.