Figure 2.

An example of results returned by the substrate analysis service. Alignment of homologues of alpha-2-antiplasmin showing cleavage at residue 30 by fibroblast activation protein alpha subunit (S09.007). This is the fifth result from the list shown above. The known cleavage occurs in the human protein (UniProt: P08697; 27) and is highlighted in green. Four residues either side of the cleavage site are highlighted. A conserved residue is highlighted in pink. A residue that differs from that in the human sequence is highlighted in orange if the same amino acid is known to occupy the same binding site from another substrate of this peptidase; and in black if the amino acid is not known to occur in the same binding site from any substrate for this peptidase. The high number of residues highlighted in black indicates that this cleavage is unlikely to be physiologically relevant, but note that the number of cleavages known for this peptidase is low.