Table 1. Experimental validation of candidate cancer genes.
| Experimental validation | Candidate cancer genes (n) | Publications (n) |
|---|---|---|
| Gene overexpression | 60 | 74 |
| Transient RNA interference | 58 | 52 |
| Mutagenesis | 31 | 41 |
| Immunostaining | 25 | 26 |
| Stable gene knockout | 23 | 22 |
| Survival analysis | 20 | 21 |
| Protein activity assay | 19 | 20 |
| Drug response assay | 15 | 17 |
| In silico protein modelling | 12 | 14 |
| Xenograft | 10 | 11 |
| Rhotekin pull-down | 2 | 5 |
| Total | 275 (120 unique genes) | 303 (166 unique publications) |
For each type of experimental validation, the numbers of validated candidate genes and corresponding publications are shown. The complete gene list with references to the original papers is given in Supplementary Table S3.