Fig. S4.

Comparison with PacBio long reads supports the accuracy of the H. dujardini genome assembly. A heat map shows the concordance between the PacBio and Illumina assemblies. The independently sequenced PacBio sequences are highly similar to the Illumina assembly, and there is no significant off-diagonal homology that would indicate misassembly or residual heterozygosity. Both sets of contigs are highly congruent. Synteny within contigs is clearly preserved, and assemblies are 98.53% concordant per base.