Fig. S4.
(A) Absorption (A, solid line), fluorescence (Fem, dashed line), and CD (θ, dotted line) spectra of native PCB-ApcEΔΔ in KPB. (B) Absorption spectra of PCB-ApcEΔΔ in 8 M acidic urea (pH 1.5). (Inset) Zinc-induced fluorescence of the SDS/PAGE indicating covalently attached chromophores. Samples were reconstituted in E. coli (Table S5), purified by Ni2+ affinity chromatography, and then dialyzed against KPB (20 mM, 0.15 M NaCl, pH 7.2) containing 0 M (thick lines) and 4 M (thin lines) urea. Emission spectra were obtained by excitation at 580 nm for PCB.