Table S5.
Plasmids used
| Antibiotic resistance | pACYCDuet derivative | pET30 or pET28 derivatives |
| Kanamycin | pET-apcE(1–240/Δ87–130) | |
| pET-apcE(1–240/Δ77–153) | ||
| pET-apcE(20–240/Δ87–130) | ||
| pET-apcEΔ | ||
| pET-apcEΔΔ | ||
| pET-apcE(36–240/Δ77–153) | ||
| pET-apcE(50–240/Δ77–153) | ||
| pET-E39A | ||
| pET-L40S | ||
| pET-D41A | ||
| pET-E42A | ||
| pET-L43S | ||
| pET-E39A/E42A | ||
| pET-R53A | ||
| pET-I75A | ||
| pET-N154R | ||
| pET-A156Q | ||
| pET-K157L | ||
| pET-K157T | ||
| pET-S158A | ||
| pET-S158C | ||
| pET-S158C/C196S | ||
| pET-R160A | ||
| pET-R160S | ||
| pET-D161A | ||
| pET-D161H | ||
| pET-W164A | ||
| pET-W164Y | ||
| pET-F165Y | ||
| pET-Y168L | ||
| pET-N184G | ||
| pET-L188G | ||
| pET-C196S | ||
| pET-A200F | ||
| pET-F216A | ||
| pET-E221A | ||
| Chloramphenicol | pACYC-ho1-pcyA |
The pACYCDuet and pET30 or pET28, from Novagen, are T7 promoter expression vectors. The pACYCDuet is designed to coexpress two target proteins in E. coli. Using the two vector derivatives, together with compatible replicons and antibiotic resistance, three proteins could be coexpressed in the same cell, thereby generating the respective designed core-membrane–like variants in E. coli.