Fig. S1.

RNA-seq analysis of HeLa extracts. (A) Abundance of mRNAs measured by RNA-seq of S10 extract incubated for 1 h under the reaction conditions (Methods) versus matching naїve extract. (B) Abundance of mRNAs measured by RNA-seq of S10 extract cleaved by endogenous RNase L versus matching control extract incubated without 2–5A. (C) Abundance of mRNAs measured by RNA-seq of S10 extract treated with recombinant RNase L for 5 s versus naїve extract. (D) Correlation of AUUUA and UAUAU abundance in the transcripts covered by the RNA-seq analysis. (E) Plain count of UAUAU sites in mRNAs from HeLa S10 samples as a function of read loss upon cleavage by RNase L. Note the log10 y axis scale.