Fig. S4.

Profiling mRNA targets of RNase L in live HeLa cells using RNAi. (A) Expression of RNase L in HeLa cells transfected with RNAi for RNase L and with scrambled RNA control (scrRNA), monitored by qPCR. (B) BioAnalyzer analysis of 28S rRNA cleavage in cells with RNase L KD. Specific cleavage bands of RNase L are marked with an asterisk. RNase L was activated by transfecting poly-I:C (Methods). (C) Signed P′ analysis of the RNA-seq data. Two biological replicates (lines 5 and 7; lines 6 and 8; Table S1) were used for the analysis. (D) GSEA preranked enrichment analysis of the RNA-seq data, conducted as in Fig. 2C.