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. 2015 Dec 14;112(52):16030–16035. doi: 10.1073/pnas.1501731113

Fig. 2.

Fig. 2.

Biophysical and pharmacological properties of the Aplysia HCN channel expressed in Xenopus laevis oocytes. (A and B) Inhibition of the acHCN channel by low concentrations of Cs+. (A) Ih current traces generated upon hyperpolarization from −30 mV to −110 mV in media with 2 mM Cs+ (red) or following washout (blue) were superimposed on the current trace generated under the same conditions in media without cesium (black). (B) I/V relationships for acHCN-mediated Ih in control conditions (black), in the presence of 2 mM Cs+ (red) or following washout (blue). The currents were normalized to the current value at −110 mV under the control conditions. Applying 2 mM Cs+ decreased Ih amplitude by 79.9 ± 2.7%, n = 4. (C and D) Applying 100 μM ZD7288 decreased the amplitude of Ih by 83.9 ± 2.4%; n = 4. (E and F) Applying 1 mM 8-Br-cAMP increased Ih amplitude by 18.0 ± 0.7%; n = 4. (G and H) Applying 1 mM 8-Br-cGMP increased Ih amplitude by 35.7 ± 20.3%; n = 4.