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. 2015 Dec 14;112(52):16030–16035. doi: 10.1073/pnas.1501731113

Fig. 4.

Fig. 4.

ZD7288 reduces behavioral conditioning but not intermediate-term sensitization or baseline responding in the siphon-withdrawal preparation. (A) The preparation. (B) Behavioral protocols. See the text for details. (C1) Examples of siphon withdrawal before (pretest) and 2.5 min after (posttest) tail shock (sensitization). (C2) Average results from experiments like the ones shown in C1 with the abdominal ganglion bathed in normal saline (control, n = 6) or ZD7288 (n = 5). The amplitude of siphon withdrawal has been normalized to the average value on the three pretests in each experiment. The overall average pretest value was 2.3 mm, not significantly different in experiments with ZD7288. The average response to the tail shock was 5.4 mm. (D1) Examples of siphon withdrawal before (pretest) and 45 min after (posttest) conditioning. (D2 and D3) Average siphon withdrawal in groups that received paired or unpaired training with the abdominal ganglion bathed in either normal seawater (control, n = 5 paired and 8 unpaired), ZD7288 (n = 6 paired and 8 unpaired), APV (n = 7 paired and 7 unpaired), or ZD7288 and APV (n = 6 paired and 5 unpaired). Responses have been normalized to the value on the pretest in each experiment. The overall average pretest value was 3.1 mm and the average response to the first tail shock US was 6.8 mm, not significantly different in experiments with ZD7288 or APV. Error bars indicate SEMs, *P < 0.01 for the difference between the paired and unpaired groups, #P < 0.05 and +P < 0.05 one tail for the reduction of that difference by each drug, and xP < 0.05 one tail for the APV × ZD7288 × pairing interaction. (D2) Average withdrawal on each test with the ganglion bathed in seawater or ZD7288. (D3) Average withdrawal overall illustrating occlusion of APV and ZD7288. (E) ZD7288 does not affect baseline evoked firing of LFS siphon motor neurons or LE siphon sensory neurons. (E1) Example of spikes in an LFS motor neuron and an LE sensory neuron evoked by a tap to the siphon. (E2 and E3) Average number of evoked spikes in LFS neurons and LE neurons with the abdominal ganglion perfused with seawater (control, n = 6) or ZD7288 (n = 5) after the third test. The number of spikes has been normalized to the average value on the three pretests in each experiment. The overall average pretest value was 15.7 for LFS neurons and 4.0 for LE neurons, not significantly different in experiments with ZD7288.