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. 2015 Dec 14;112(52):16042–16047. doi: 10.1073/pnas.1514250112

Fig. 1.

Fig. 1.

Musclin expression is exercise-responsive. Musclin expression was tested in muscles of WT mice after 5 d of treadmill exercise vs. no exercise (control). (A) Representative Western blots for musclin and GAPDH in protein extracts from gastrocnemius. (B–D) Summary statistics for musclin protein expression normalized to GAPDH by densitometry of Western blots of protein extracts from gastrocnemius (B), tibialis anterior musclin mRNA normalized to hypoxanthine guanine phosphoribosyl transferase (HPRT) by quantitative RT-PCR (qRT-PCR) (C), and musclin peptide expression in plasma by custom ELISA (the y axis range begins at the lower limit for detection for this assay of 20 pg/mL; D). (E) Representative immunohistochemical stains of gastrocnemius cross-sections imaged by confocal microscopy. Red, musclin; green, nuclei. *P < 0.05 vs. control.