Skip to main content
. 2015 Dec 14;112(52):16042–16047. doi: 10.1073/pnas.1514250112

Fig. 5.

Fig. 5.

Musclin augments ANP signaling in skeletal muscle. Mice were exercised on a treadmill for 5 d before undergoing assessment of ANP signaling. (A) Summary statistics for plasma ANP as assessed by ELISA. (B) Summary statistics for cGMP in gastrocnemius by enzyme immunoassay. *P < 0.05 Ostn-KO vs. WT. (C) Summary statistics for cGMP production in a culture of murine primary myoblasts exposed to various concentrations of ANP and musclin. *P < 0.05 vs. columns indicated by bar. (D) Summary statistics for percent increase in peroxisome proliferator-activated receptor gamma coactivator 1-α (PGC1-α) mRNA over baseline in response to exercise in tibialis anterior by qRT-PCR. *P < 0.05 vs. WT. (E) Summary statistics for primary myoblast culture mRNA of PGC1-α normalized to HPRT in response to musclin/ANP or to 8-Br-c-GMP. (F) Summary statistics for primary myoblast culture relative mRNA of TFAM, NRF1, and NRF2 with and without musclin/ANP. KO, Ostn-KO.