Table S1.

Body size, hind-limb bone thickness and length, body composition, and basic hemodynamic properties in mice

Physiologic parameter	WT	n	Ostn-KO	n	P
Muscle mass GCN, mg	84.3 ± 7.0	11	93.5 ± 6.1	12	0.33
Muscle mass QDR, mg	97.6 ± 9.7	11	101.0 ± 7.2	12	0.80
Body length, cm	9.3 ± 0.1	5	9.2 ± 0.2	5	0.41
Fore-limb length, cm	2.1 ± 0.02	5	2.2 ± 0.04	5	0.61
Hind-limb length, cm	2.7 ± 0.04	5	2.6 ± 0.06	5	0.80
Systolic BP, mmHg	110 ± 2	5	113 ± 2	5	0.33
Diastolic BP, mmHg	63 ± 2	5	61 ± 2	5	0.72
Heart rate, beats per min	516 ± 10	5	509 ± 14	5	0.66
Hind-limb CT data
Right femur mean cortical thickness, mm	0.24 ± 0.02	3	0.24 ± 0.004	3	0.75
Left femur mean cortical thickness, mm	0.23 ± 0.004	3	0.23 ± 0.004	3	1
Right tibia mean cortical thickness, mm	0.25 ± 0.007	3	0.25 ± 0.01	3	0.87
Left tibia mean cortical thickness, mm	0.25 ± 0.008	2	0.24 ± 0.003	3	0.47
Right femur max cortical thickness, mm	0.30 ± 0.03	3	0.31 ± 0.003	3	0.85
Left femur max cortical thickness, mm	0.30 ± 0.003	3	0.31 ± 0.01	3	0.29
Right tibia max cortical thickness, mm	0.37 ± 0.009	3	0.35 ± 0.02	3	0.4
Left tibia max cortical thickness, mm	0.36 ± 0.03	2	0.33 ± 0.005	3	0.35
Right femur length, mm	14.46 ± 0.01	3	14.13 ± 0.20	3	0.19
Left femur length, mm	14.46 ± 0.05	3	15.30 ± 1.06	3	0.47
Right tibia length, mm	17.44 ± 0.16	3	17.06 ± 0.07	3	0.10
Left tibia length, mm	17.25 ± 0.15	2	17.03 ± 0.07	3	0.23
TD-NMR data
Body weight, g	21.2 ± 0.5	15	21.9 ± 0.1	26	0.36
Fat, g	2.5 ± 0.1	15	2.6 ± 0.1	26	0.54
Lean, g	16.0 ± 0.4	15	16.2 ± 0.4	26	0.74
Fluid, g	2.7 ± 0.1	15	2.8 ± 0.1	26	0.62
% fat	11.9 ± 0.6	15	12.0 ± 0.5	26	0.88
% lean	75.5 ± 0.5	15	73.9 ± 0.3	26	0.01
% fluid	13.0 ± 0.3	15	12.8 ± 0.3	26	0.67

Left lower leg motion artifact prevented tibial measurements in one WT mouse. BP, blood pressure; GCN, gastrocnemius muscle; QDR, quadriceps femoris muscle.