Table S1.

High-throughput MARSALA sequencing data for case 1

Sample	Raw data		Clean data			Mapped ratio, %	Coverage ratio, %	Coverage depth, ×	PCR coverage, number of reads (range)	Mutation coverage, number of reads
	Reads, M	Bases, G	Reads, M	Bases, G	Ratio, %
Husband	2.37	0.36	2.36	0.33	99.8	90.8	7.2	0.12	93,565 (89,880–179,880)	45,626
Wife	2.26	0.34	2.26	0.32	99.8	93.4	6.9	0.11	90,747 (87,057–174,597)	88,343
Embryo 1	57.03	5.76	47.18	4.14	71.8	74.5	44.0	1.08	78,724 (67,338–84,378)	78,986
Embryo 2	1.95	0.30	1.78	0.24	80.7	86.7	4.9	0.08	4,988 (4,631–9,510)	4,807
Embryo 3	1.88	0.28	1.71	0.23	79.4	87.9	4.1	0.07	138,376 (132,250–267,006)	134,373
Embryo 4	2.37	0.36	2.19	0.29	81.7	88.2	5.2	0.10	98,989 (94,862–190,982)	96,087
Embryo 5	61.95	6.26	51.54	4.53	72.3	80.6	48.6	1.28	101,268 (89,705–108,092)	53,175
Embryo 6	2.17	0.33	2.05	0.28	85.4	88.4	4.8	0.09	113,555 (108,469–217,346)	68,570
Embryo 7	69.80	7.05	56.76	5.04	71.5	80.0	44.7	1.41	73,399 (63,270–78,426)	73,907
Embryo 8	1.92	0.29	1.78	0.24	81.2	86.4	4.3	0.08	85,301 (81,715–164,500)	126,922
Embryo 9	2.00	0.30	1.84	0.25	81.1	82.3	4.5	0.08	112,544 (107,858–214,737)	103,694
Embryo 10	1.54	0.23	1.48	0.22	82.4	87.9	4.0	0.06	74,050 (70,783–141,802)	60,681
Embryo 11	64.19	6.48	54.35	4.81	74.3	79.1	48.0	1.33	76,183 (70,044–80,863)	11,244
Embryo 12	62.81	6.34	50.81	4.51	71.1	74.8	39.5	1.18	86,191 (75,850–91,089)	70,031
Embryo 13	53.78	5.43	46.37	4.12	75.8	83.1	45.8	1.20	95,731 (81,121–102,536)	96,351
Embryo 14	2.13	0.32	1.89	0.25	76.0	85.5	5.0	0.08	72,325 (68,956–139,359)	70,067
Embryo 15	63.30	6.39	54.85	4.95	77.4	85.0	48.9	1.47	93,407 (80,412–99,998)	94,364
Embryo 16	2.18	0.33	1.82	0.23	69.6	87.7	5.0	0.08	11,348 (10,444–20,997)	4,421
Embryo 17	2.35	0.36	2.09	0.27	76.6	83.9	5.3	0.09	101,793 (97,353–195,010)	98,741
Embryo 18	1.63	0.25	1.47	0.19	78.3	87.2	4.0	0.06	67,031 (61,240–128,853)	64,964