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. 2015 Dec 14;112(52):E7230–E7238. doi: 10.1073/pnas.1507459112

Fig. 5.

Fig. 5.

CARD11(L225LI)-transformed cells are sensitive to inhibition of NF-κB or JNK signaling. (A) Survival and proliferation of CD19-Cre and CARD11(L225LI)CD19-Cre splenocytes was evaluated by a Trypan Blue exclusion assay. The experiment was conducted twice, with a total n = 7. CD19-Cre MACS-purified B cells and CARD11(L225LI)CD19-Cre splenocytes were treated with solvent control DMSO and (B) treated with 10 μM Bay11-7082 and (C) with 20 μM SP600125 for 4 h, and nuclear lysates were examined for the presence of (B) NF-κB subunits and (C) AP-1 subunits. Representative results from six experiments are shown. (D–I) Cells were cultured with solvent control DMSO or in the presence of the indicated inhibitors and concentrations for 48 h. Annexin V- and 7AAD-double-negative cells were determined cytometrically. The experiment was performed at least twice, with n ≥ 4. The data are presented as the mean ± SEM.