Fig. S1.

Effects of CARD11(L225LI) in vitro. (A) Bal-17 cells were infected with retroviruses to express empty vector (GFP), CARD11 WT, and CARD11(L225LI) together with GFP. Activation marker expression (CD80, CD86, and IgM) was measured using flow cytometry. Live cells were further gated according to GFP expression. (B) GFP-expressing cells were FACS-sorted and expanded; the cells were left either unstimulated or stimulated with 10 μg/mL αIgM for 90 min, as indicated. Nuclear extracts were prepared and subjected to a gel mobility shift assay for NF-κB DNA-binding activity. The data shown are representative of at least two independent experiments.