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. 2015 Dec 11;112(52):16048–16053. doi: 10.1073/pnas.1521675112

Fig. 4.

Fig. 4.

Increased transcription of pectin methylesterase inhibitors and the restoration of pectin esterification to wild-type levels in mon1 cob-6 and sfr6-3 cob-6. Bar graphs indicate means ± standard deviation. Letters above the bars indicate significant differences based on one-way ANOVA and Tukey's test, P < 0.05. (A) Venn diagram for RNA-seq analysis of cob-6, sfr6-3, and sfr6-3 cob-6 shows all genes that are up-regulated and down-regulated compared with wild type with 1.5-fold change or above. For further analysis, we looked at genes that are misexpressed between sfr6-3 cob-6 and cob-6, and identified 48 cell wall-related genes. (B) Pectin esterification levels in the different lines. Values were normalized to the total amount of galacturonic acid (Fig. S7A). (C) Steady-state mRNA levels of two pectin methylesterase inhibitor genes, AT3G17130 (PMEI8) and AT1G62770 (PMEI9), in various genotypes. (D) PMEI8 and PMEI9 were overexpressed (OX) in the cob-6 background. Ten independent lines were analyzed, all demonstrating partial suppression of the cob-6 phenotype.