Fig. S5.

Conditional expression of IL-1β in lungs promotes neutrophil recruitment, Tsp-1 degradation, and enhanced metastatic outgrowth. (A) Representative scatter plots depicting flow cytometry analysis of Ly6G⁺ cells in lungs of CCSP-rtTA; tetO–IL-1β or age-matched control littermates (WT or CCSP-rtTA single-transgenic) administered doxycycline for 10 d. (B) Quantitation of number of surface metastases in lungs 28 d after tail vein injection of 1 × 10⁶ mouse mammary tumor virus-polyoma middle T antigen (MMTV-PyMT) (FVB/N strain)–derived breast cancer single-cell suspensions into syngeneic CCSP-rtTA; tetO–IL-1β or age-matched control littermates (FVB/N strain) that were administered doxycycline for 8 d. Data are plotted as the mean ± SEM (n = 7 controls and n = 10 CCSP-rtTA; tetO–IL-1β). *P = 0.018 (one-tailed t test with Welch’s correction).