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. 2015 Dec 14;112(52):15982–15987. doi: 10.1073/pnas.1514265112

Fig. 2.

Fig. 2.

Maize inbreds exhibiting substantial differences in chiasma number have similar synaptonemal complex (SC) length. (AC) Immunolocalization of the ZYP1 protein to measure the SC length in maize. (A) Green, ZYP1 immunolocalization; red, DAPI-stained chromatin. (B) Green, ZYP1 immunolocalization. (C) Measurement of the SC length. The total length of synaptonemal complex in the nucleus was determined by tracing the extent of the ZYP1 protein (white) using the Distance Measurement tool in the softWoRx software. (Scale bar, 5 μm.) (D) Comparison of the SC length and chiasma number in the A344 and CML228 inbreds. Bars = ±SD.