Fig. 3.

CO numbers vary coordinately with DSB number in maize. (A and B) Immunolocalization of RAD51 in zygotene meiocytes in B73 (A) and CML228 (B). Green, RAD51 immunolocalization; red, DAPI-stained chromatin. Images are flat projections of three consecutive Z sections out of a total of 80–90 Z sections through the entire nucleus. (Scale bar: 5 μm.) (C) Variation in the mean number of RAD51 foci per cell in male meiocytes at midzygotene. Each blue circle represents a RAD51 focus count from a single meiocyte. Darker color represents multiple cells that have similar RAD51 foci numbers. Bars = ±SE. C.V., coefficient of variation. (D) Comparison of chiasma numbers and the numbers of RAD51 foci in six maize inbred lines. Bars = ±SD.