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H3K4me3 localization is not a good predictor of meiotic recombination sites in maize. (A) Dynamics of H3K4me3 during meiosis in maize. H3K4me3 foci first appear during leptotene and persist throughout meiosis I. Green, H3K4me3 immunolocalization; red, DAPI-stained chromatin. (Scale bar: 5 μm.) (B) Colocalization of H3K4me3 and RAD51 at zygotene in the B73 inbred. Green, RAD51 immunolocalization; red, H3K4me3 immunolocalization; blue, DAPI-stained chromatin. (Scale bar: 5 μm.)
