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. 2015 Dec 14;112(52):E7249–E7256. doi: 10.1073/pnas.1516305112

Fig. 2.

Fig. 2.

Specific activation of FLuc expression in HeLa cells by sgRNAs targeting A3B or A3G promoter sequences. The A3G- and A3B-derived promoter sequences targeted in these assays are shown in Fig. 1. (A) A3G sgG1 and sgG2 activate FLuc expression from a reporter plasmid containing the targeted region of the A3G promoter (A3G rep1) but do not activate a reporter containing the homologous region from the A3B promoter (A3B rep1). (B) A3B sgB1 specifically activates the targeted region of the A3B promoter (A3B rep2) and does not activate via the homologous region of the A3G promoter (A3G rep2). (C) A3B sgB2 strongly activates the targeted region of the A3B promoter (A3B rep3) and modestly activates the homologous region of the A3G promoter (A3G rep3). Data shown represent the average of three independent experiments with SD indicated. Note that the A3G rep1 indicator construct contains both targeted regions of the A3G promoter, whereas the two regions analyzed in the A3B promoter are present in two distinct indicator plasmids.