Fig. 3.

TET2 differentially affects Z versus R transactivation of lytic EBV promoters. The ability of Z and R to induce expression of unmethylated, methylated, and 5-hydroxymethylated lytic viral promoters was assessed by luciferase assays. EBV promoter-luciferase constructs (containing various lytic EBV promoters inserted upstream of the luciferase gene in a CpG-free vector) were methylated or mock-treated in vitro using M.SssI as indicated and transfected into EBV-negative HONE.1 cells with either vector controls, a Z expression vector (with or without a TET2 expression vector) (A), or an R expression vector (with or without a TET2 expression vector) (B). Luciferase assays were performed 2 d posttransfection; the fold luciferase activation is shown relative to the activity of the unmethylated promoter transfected with control vectors (set at 1). The error bars indicate +1 SD calculated from three replicate experiments.