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(A) Two models for Puf2–RNA interactions. (B) Predicted interactions given the two models. (C and D) Compensatory mutants in the yeast three-hybrid assay show one Puf2p binds one UAAU site, with R1 contacting the first U. Nucleotides differing from the WT RNA sequence are in red, and all UAAU and UAAG sequences are highlighted.
