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. 2015 Dec 16;5(12):150171. doi: 10.1098/rsob.150171

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© 2015 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 1. — Profile of anti-Mtb antibodies in the donor sera. Panel (a) shows Ab titres against membrane and cytosolic antigens, determined by ELISA. Median, interquartile range and individual OD values of five donor sera (diluted 1 : 400) are shown. Panel (b) depicts immunoblotting of membrane antigens with the mAb CS-35 showing presence of LAM (lane 1) and with donor sera (lanes 2–6) showing Abs to various antigens including the characteristic band of LAM. Panels (c) and (d) show, respectively, competitive binding of serum Abs (mean OD ± s.e. of five sera) to the membrane antigens LAM and Acr in presence of mAbs CS-35 and IT-4. *p < 0.05, **p < 0.02, ***p < 0.01.