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. 2015 Dec 16;5(12):150171. doi: 10.1098/rsob.150171

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© 2015 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 5. — Opsonization with serum Abs enhanced colocalization of Mtb with Lysotracker Red. Cells were allowed to internalize bead or Mtb (HK/UNOP/OP) followed by labelling of acidified compartments with Lysotracker Red. Panel (a) shows representative images (with 40× objective), including enlarged view of one cell (indicated by arrows) displayed in the insets. Scale bar is 10 µM. Panel (b) shows whole-cell intensity of Lysotracker Red under different conditions. Box and whisker plot (median and 5th–95th percentile values) represents pooled data of 500 cells (100 cells from each donor, each cell with at least one particle). Panel (b) shows colocalization of Lysotracker Red with the bacilli, measured as Mander's coefficient. The box and whisker plot represents median and 5th–95th percentile values from pooled data of 250 cells (50 cells from each donor, each cell with at least one bacillus). The p-values are also depicted in panels (b) and (c).