Figure 5.

Disinhibition of WWP2 by Dvl2 depends on its polymerization. (a) Western blot of His pull-downs from HEK293T cells co-transfected with His-Ub, HA-WWP2 and wt or polymerization-deficient Flag-Dvl2 mutants (M2, M4), as indicated above panel; note the complete lack of activity of these mutants in disinhibiting WWP2. This is not due to lack of phosphorylation, because a phosphorylation-deficient DEP domain mutant (E499G) is nearly fully active. (b) Western blots of coIPs from HEK293ET cells co-transfected with catalytically inactive WWP2 and wt or mutant Flag-Dvl2, demonstrating that lack of polymerization does not affect the binding of Dvl2 to WWP2.