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. 2015 Dec 23;5(12):150209. doi: 10.1098/rsob.150209

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© 2015 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 3. — Plk4 over-expression leads to loss of hair and its pigmentation. (a) Plk4^OE/Plk4^OE; p53^KO/p53^KO mouse exhibiting typical hair loss phenotype. (b) Plk4^OE/Plk4^OE; p53^KO/p53^KO mice showing varying degrees of greying hair. (c) Q-RT-PCR analysis of Plk4 and tyrosinase transcripts in extracts of total back skin from mice of indicated genotypes and treatment indicating mean ± s.e. for three independent biological samples and three replicates/sample (+DOX indicates doxycycline treatment). (d) Fontana-Masson staining of cryosections of P2 back skin from mice of indicated genotypes. +DOX indicates treatment with doxycycline. (e) Fontana-Masson staining of P20 back skin cryosections from mice of indicated genotypes and treated with doxycycline where indicated (+DOX). (f) Anagen hair follicle in wild-type back skin immunostained to reveal melanocyte markers KIT (red in merge; white, monochrome), MITF (green) and DCT (blue in merge; white in monochrome). DNA is represented in white. Differentiated melanocytes identified by triple KIT⁺MITF⁺DCT⁺ staining. (g) P2 back skin from Plk4^OE/Plk4^OE mice without or with Plk4 over-expression (+DOX) showing anagen hair follicle immunostained to reveal melanocyte markers KIT (red in merge; white in monochrome), MITF (green) and DCT (blue in merge; white in monochrome). DNA is white in merge. Note reduction in differentiated melanocytes identified by triple KIT⁺MITF⁺DCT⁺-positive immunostaining. (h) P2 back skin from Plk4^OE/Plk4^OE; p53^KO/p53^KO mice without or with Plk4 over-expression (+DOX) showing hair follicles immunostained for melanocyte markers KIT (red in merge; white in monochrome), MITF (green) and DCT (blue in merge; white in monochrome). DNA is white in merge. Differentiated melanocytes (KIT⁺MITF⁺DCT⁺) are reduced in Plk4^OE/Plk4^OE; p53^KO/p53^KO (+DOX) mice. (i) Mean numbers ± s.d. of differentiated melanocytes (KIT⁺MITF⁺DCT⁺) per bulb in indicated genotypes and treatments. Significance determined by Student's t-test. *p < 0.05, **p < 0.01 and ***p < 0.005.