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. 2016 Jan 7;118(1):38–47. doi: 10.1161/CIRCRESAHA.115.307408

Figure 2.

Figure 2.

Inhibition of microRNA-17–92 cluster decreases proliferation and angiogenic sprouting in endothelial cells. Human umbilical vein endothelial cells (HUVECs) were transfected with 70 nmol/L mix of miR-17–92 inhibitors (Inh-miR-17–92) or control inhibitor (CI). A, Quantitative reverse transcription polymerase chain reaction analysis of individual miR-17–92 cluster members analyzed 36 hours post-transfection. Data are expressed as relative miRNA levels vs CI. B, Total number of cells, 24 or 48 hours post-transfection. C–G, Fibrin gel bead assay was performed 36 hours post-transfection. HUVECs were allowed to undergo morphogenesis for 2 days in the presence of EGM-2 (endothelial cell growth factor medium 2) media containing VEGF (vascular endothelial growth factor). C, Representative images of HUVECs bead spheres. D–G, Quantification of angiogenic sprouting: (D) cumulative sprout length, (E) number of sprouts, (F) number of branch, and (G) number of detached cells. Data are expressed as mean±SEM of 3 independent experiments. *P≤0.05, compared with cells transfected with CI (Scale, 84 μm).