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. Author manuscript; available in PMC: 2016 Jan 6.
Published in final edited form as: ACS Chem Biol. 2015 Sep 4;10(11):2589–2597. doi: 10.1021/acschembio.5b00466

Figure 1. AGPS functional role and inhibition of AGPS activity by lead inhibitors.

Figure 1

(A) AGPS catalyzes the formation of alkyl-DHAP from displacement of the acyl group by a fatty alcohol from the substrate acyl-DHAP. The enzyme is located in the peroxisomes. (B) Inhibition of Cavia porcellus AGPS activity was assessed by a radioactivity assay using 100 μM palmitoyl-DHAP, 100 μM [1-14C]hexadecanol, 180μM inhibitor and detecting the formation of [1-14C]hexadecanyl-DHAP as function of time. The controls were performed using AGPS alone and the catalytically inactive AGPS mutant Thr578Phe. Measurements were performed at least in triplicate [8].