. Author manuscript; available in PMC: 2016 Dec 21.

Published in final edited form as: Chem Res Toxicol. 2015 Dec 3;28(12):2400–2410. doi: 10.1021/acs.chemrestox.5b00405

Table 3.

Metabolism of AαC and Other Heterocyclic Amines by Hepatic Enzymes^a

	P450 Oxidation (pmol/min/mg protein)				FMO activity (nmol/min/mg protein)
	AαC	PhIP	MeIQx	MROD	Methimazole	AaC

WT mouse liver microsomes	103 ± 33	362 ± 120	404 ± 155	115 ± 23	2.90 ± 0.67	N.D.
LCN mouse liver microsomes	N.D.	N.D.	67 ± 44	3.8 ± 1.0	3.97 ± 0.80	N.D.
Human liver microsome (H112)	820 ± 60	1440 ± 280	1010 ± 155	90.1 ± 2.5
	Xanthine Dehydrogenase (nmol/min/mg protein)				Aldehyde Oxidase (nmol/min/mg protein)
	Xanthine		AαC		DMAC	AαC

WT mouse liver cytosol	2.12 ± 0.48		N.D.		783 ± 93	N.D.
LCN mouseliver cytosol	1.72 ± 0.37		N.D.		592 ± 148	N.D.

Enzyme assays were carried out as described in Methods. Values are reported as the mean and SD of at least 3 mice (n ≥3). Substrate concentrations assayed were: 10 μM (MROD); 25 μM (DMAC), 50 μM (Xanthine); 100 μM (AαC, PhIP, MelQx); and 1 mM (Methimazole). N.D., not detected; < 0.05 pmol AαC metabolized/min/mg protein